Ettan dige imager manual

fluorescent excitation and emission filters and are matched to each other through imaging software. DeCyder™ 2-D Differential Analysis Software (DeCyder 2D) v is specifically designed for 2-D Fluorescence Difference Gel Electrophoresis (2-D DIGE) analysis and is a key element in the DIGE system. Unlike other 2-D. 2-DIGE overlay image of samples labelled with Cy3 and Cy5. 2-D gel separation of a sample pre-labelled with CyDye 30 25 20 15 10 5 0 DIGE Spot, CV ranking Ettan DALT systems DIGE Gel and Buffer Kit CyDye DIGE Fluor Minimal Dyes Our products reduce manual labor, and work together. Imaging systems using laser light excitation or other visible light source excitation are not recommended for imaging gels stained with Oriole fluorescent gel stain. These include PharosFX™ system (Bio-Rad), Typhoon, Storm, and Ettan DIGE Imager (GE Healthcare), Odyssey (Li-Cor), FLA (Fuji), and FM Bio (Hitachi) systems.

Typhoon ™ scanner (GE Healthcare); alternative equipment is FLA Imaging System (FUJIFILM) or Ettan DIGE imager (GE HealthCare) Ettan™ Spot Handling Workstation (GE HealthCare). 2 AA DIGE Gel is intended for use with the DIGE Buffer Kit and Ettan DALT Large Vertical System. Ettan DALTtwelve and Ettan DALTsix instruments are designed for large-format, second dimension 2-D electrophoresis using either 18 or. Ettan DIGE system is a fully integrated system offering significant benefits over traditional 2-D electrophoresis. It includes Typhoon™ Variable Mode Imager, DeCyder™ Differential Analysis Software, and CyDye DIGE Fluor dyes. In 2-D DIGE, samples are prelabelled with one of the CyDye DIGE fluors and multiplexed within the same gel.

enables detection, quantitation, matching and analysis of Ettan DIGE system gels. Whenever “DeCyder 2D Software” is used in this manual, it implies DeCyder 2D version software. The software was developed as part of Ettan DIGE system, to exploit the multiplexing capabilities of the CyDye DIGE Fluor dyes. Figure Legend Snippet: 2D-DIGE gel images. (A) Representative 2D image (scanned using an Ettan DIGE Imager, GE Healthcare) of a Cy2-labeled mixture of the 48 protein extracts (50 μg of each). The total number of polypeptide spots observed and matched is (only polypeptide. Ettan DALTtwelve, or Ettan DALTsix vertical electrophoresis systems for second-dimension separation; Typhoon series Variable Mode Imager for advanced imaging; and DeCyder 2-D Differential Analysis Software for quantitation and statistical analysis of protein differences. More detailed information and protocols for working with Ettan DIGE.