Gel extraction qiagen manual

Gel purification of DNA is a common technique for the isolation of specific fragments from reaction mixtures. However, most methods either fail to completely remove agarose or shear DNA which can lead to problems in downstream manipulations. The E.Z.N.A.® Gel Extraction Kit uses proprietary chemistry and HiBind® technology to recover DNA. Sample amount. gel. Fragment size. 50 bp−approx. 20 kbp. Typical recovery. 70−95 %. Theoretical binding capacity. 25 . mdi Gel Extraction Kit is a quick, convenient and economical tool to isolate DNA fragments (70bpkb) after gel analysis from standard or low melt Agarose gel. The buffer system provided in the kit facilitates efficient binding of DNA fragments from solubilized gel slice on spin column.

MinElute PCR Purification Kitsfor direct purification of double-stranded PCR products (70 bp - 4 kb) from amplification reactions. MinElute Gel Extraction Kitsfor extraction of DNA fragments (70 bp - 4 kb) from standard or low-melt agarose gels in TAE (Tris-acetate/EDTA) or TBE (Tris-borate/EDTA) buffer. The E.Z.N.A.® Gel Extraction Kit is available with two different types of columns. V-spin columns have an attached cap, while Q-spin columns are capless. The columns are otherwise identical in use and application. Either column can be used with either the vacuum or centrifugation protocols. D is the V-spin version of the Gel Extraction Kit. PCR Purification Nucleotide Gel Extraction Kit Removal Kit Kit Maximum binding capacity: 10 µg 10 µg 10 µg Maximum weight of gel slice: — mg Minimum elution volume: 30 µl 30 µl 30 µl Capacity of column reservoir: µl µl µl Typical recoveries Recovery of DNA: % % %.

PCR Purification Nucleotide Gel Extraction Kit Removal Kit Kit Maximum binding capacity: 10 µg 10 µg 10 µg Maximum weight of gel slice: — mg Minimum elution volume: 30 µl 30 µl 30 µl Capacity of column reservoir: µl µl µl Typical recoveries Recovery of DNA: 90–95% 80–95% 70–80%. 1. Excise the DNA fragment from the agarose gel with a clean, sharp scalpel. Minimize the size of the gel slice by removing extra agarose. 2. Weigh the gel slice in a colorless tube. Add 3 volumes of Buffer QG to 1 volume of gel ( mg ~ µl). For example, add µl of Buffer QG to each mg of gel. For 2% agarose gels, add 6 volumes of Buffer QG. The QIAquick PCR Purification Kit and QIAquick Gel Extraction Kit can be fully automated on the QIAcube. The innovative QIAcube uses advanced technology to process QIAGEN spin columns, enabling seamless integration of automated, low-throughput sample prep into your laboratory workflow. Sample preparation using the.